False negative results in the Roche assay for HDL-cholesterol

Measurement of HDL-cholesterol is important in the risk evaluation of cardiovascular disease. Recently, we encountered a 61-year-old man with a negative result for HDL-cholesterol using the Roche assay. Further analysis revealed that this patient had a monoclonal Ig type IgMκ, with an electrophoresi...

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Bibliographic Details
Published in:Annals of clinical biochemistry 2003-09, Vol.40 (5), p.572-575
Main Authors: BAKKER, Andries J, ZIJLSTRA, Appie, LEEMHUIS, Marten P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calibration
Cardiovascular Diseases - diagnosis
Cholesterol, HDL - blood
False Negative Reactions
Humans
Hypergammaglobulinemia - blood
Immunoglobulin M - blood
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Middle Aged
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reagent Kits, Diagnostic
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Summary:Measurement of HDL-cholesterol is important in the risk evaluation of cardiovascular disease. Recently, we encountered a 61-year-old man with a negative result for HDL-cholesterol using the Roche assay. Further analysis revealed that this patient had a monoclonal Ig type IgMκ, with an electrophoresis-based concentration of 55.8 g/L, which apparently caused the negative result for HDL-cholesterol. Analysis of serum samples from other patients with monoclonal or polyclonal hypergammaglobulinaemia revealed that false negative results in the Roche assay for HDL-cholesterol may occur incidentally. Since negative results are detected easily in the laboratory, such patients do not pose a problem in daily practice. However, it is reasonable to suppose that false low results could also be caused by monoclonal as well as polyclonal Igs. Since such false low results remain undetected, vascular risk estimates consequently are altered and unnecessary treatment might be started.
ISSN:0004-5632
1758-1001
DOI:10.1258/000456303322326533