Inoculation with DNA encoding the glycoprotein gp2 reduces severity of equine herpesvirus 1 infection in a mouse respiratory model

The envelope glycoprotein 2 (gp2) of equine herpesvirus 1 (EHV-1) has no known homologue in other herpesviruses with the exception of some equid alphaherpesviruses. In order to investigate the potential of gp2 as a vaccine antigen, expression vectors were constructed to encode full-length gp2, a tru...

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Bibliographic Details
Published in:Archives of virology 2003-09, Vol.148 (9), p.1805-1813
Main Authors: LEARMONTH, G. S, LOVE, D. N, GILKERSON, J. R, WELLINGTON, J. E, WHALLEY, J. M
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antibodies
Antigens
Artificial chromosomes
Biological and medical sciences
Cell culture
Equine herpesvirus 1
Female
Fundamental and applied biological sciences. Psychology
Genes
Genomes
glycoprotein gp2
Glycoproteins
Herpes viruses
Herpesviridae Infections - prevention & control
Herpesvirus 1, Equid - immunology
Herpesvirus Vaccines - immunology
Immunization
Infections
membrane anchors
Mice
Mice, Inbred BALB C
Microbiology
Polypeptides
Respiratory diseases
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - immunology
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Virology
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Summary:The envelope glycoprotein 2 (gp2) of equine herpesvirus 1 (EHV-1) has no known homologue in other herpesviruses with the exception of some equid alphaherpesviruses. In order to investigate the potential of gp2 as a vaccine antigen, expression vectors were constructed to encode full-length gp2, a truncated version lacking the membrane anchor, and the C-terminal region. Intramuscular inoculation of mice with these DNA constructs induced neutralizing antibody against EHV-1 and, following intranasal challenge with EHV-1, mice inoculated with any of the gp2 DNA constructs cleared virus more rapidly from their lungs than control mice. The rate of clearance was comparable to that for glycoprotein D DNA, indicating gp2 as a potential antigen for inclusion in a subunit vaccine.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-003-0146-1