Isolation and characterization of a Brassica napus cDNA corresponding to a B‐class floral development gene

B‐class floral homeotic genes are required for the proper formation and identity of petals and stamens in dicot flowers. A partial cDNA clone encoding a B‐class gene, BnAP3 (Brassica napus APETALA3), was isolated from a B. napus cDNA library derived from young inflorescence meristems. The 5′ region...

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Bibliographic Details
Published in:Journal of experimental botany 2003-10, Vol.54 (391), p.2385-2387
Main Authors: Pylatuik, Jeffrey D., Lindsay, Donna L., Davis, Arthur R., Bonham‐Smith, Peta C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Arabidopsis thaliana
BnAP3
Brassica napus
Brassica napus - genetics
Brassica napus - growth & development
Brassica napus - metabolism
Carpels
Cloning, Molecular
Complementary DNA
Developmental biology
DNA, Complementary - chemistry
DNA, Complementary - genetics
Flowering
Flowers - genetics
Flowers - growth & development
Flowers - metabolism
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
GENE NOTE
Genes
Homeobox genes
Key words: Floral development
Molecular Sequence Data
Petals
Plant Proteins - genetics
Plant Proteins - metabolism
Plants
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Stamens
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Summary:B‐class floral homeotic genes are required for the proper formation and identity of petals and stamens in dicot flowers. A partial cDNA clone encoding a B‐class gene, BnAP3 (Brassica napus APETALA3), was isolated from a B. napus cDNA library derived from young inflorescence meristems. The 5′ region of the cDNA was retrieved by RACE. The deduced amino acid sequence of the full‐length clone exhibited high similarity to APETALA3 of Arabidopsis thaliana and functionally homologous proteins from other species. 5′ RACE and Southern analysis suggests that BnAP3 has multiple alleles in B. napus. Expression analysis assayed by RT‐PCR shows that BnAP3 is expressed in floral tissues, as well as non‐floral tissues such as root and bract. Transformation of wild‐type A. thaliana and B. napus plants with BnAP3 under the control of a promoter specific to reproductive organs converts carpels to stamens, while the expression of this construct in A. thaliana plants mutant for AP3 restores the development of third‐whorl stamens in addition to directing a carpel to stamen conversion in the fourth whorl.
ISSN:0022-0957
1460-2431
1460-2431
DOI:10.1093/jxb/erg254