Diameter of the Cell-to-Cell Junctional Membrane Channels as Probed with Neutral Molecules

The cell-to-cell channels in the junctions of an insect salivary gland and of insect and mammalian cells in culture were probed with fluorescent molecules--neutral linear oligosaccharides, neutral branched glycopeptides, and charged linear peptides. From the molecular dimensions of the largest perme...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1981-07, Vol.213 (4507), p.551-553
Main Authors: Schwarzmann, G., Wiegandt, H., Rose, B., Zimmerman, A., Ben-Haim, D., Loewenstein, W. R.
Format: Article
Language:English
Subjects:
animal cells
Animal glands
Animals
Cell culture techniques
Cell interaction
Cell interactions
cell junctions
Cell Line
channels
Chironomidae
Cultured cells
DNA
Fluorescent Dyes
fluorescent probes
Giant cells
Glycopeptides
Glycopeptides - metabolism
insects
Intercellular junctions
Intercellular Junctions - ultrastructure
Mice
Mice, Inbred BALB C
Models, Molecular
Molecules
Oligosaccharides - metabolism
Plasmids
Protein Conformation
salivary gland
Salivary glands
Salivary Glands - ultrastructure
Species Specificity
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Summary:The cell-to-cell channels in the junctions of an insect salivary gland and of insect and mammalian cells in culture were probed with fluorescent molecules--neutral linear oligosaccharides, neutral branched glycopeptides, and charged linear peptides. From the molecular dimensions of the largest permeants and smallest impermeants the permeation-limiting channel diameter was obtained: 16 to 20 angstroms for the mammalian cells and 20 to 30 angstroms for the insect cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7244653