Vaccination with E. coli Recombinant Empty Viral Particles of Infectious Bursal Disease Virus (IBDV) Confer Protection

The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). The entire sequence region encoding VP2, VP4, and VP3 in that order was cloned and sequenced. Following subcloning into the Escherichia coli expression vector...

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Bibliographic Details
Published in:Virus genes 2003-10, Vol.27 (2), p.169-175
Main Authors: Rogel, Arie, Benvenisti, Luna, Sela, Ilan, Edelbaum, Orit, Tanne, Edna, Shachar, Yehoshua, Zanberg, Yehuda, Gontmakher, Tanya, Khayat, Eli, Stram, Yehuda
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - biosynthesis
Birnaviridae Infections - immunology
Birnaviridae Infections - prevention & control
Birnaviridae Infections - veterinary
Blotting, Western
Chickens - immunology
Cloning, Molecular
Escherichia coli - genetics
Escherichia coli - metabolism
Genetic Vectors
Infectious bursal disease virus - genetics
Infectious bursal disease virus - immunology
Microscopy, Immunoelectron
Poultry Diseases - immunology
Poultry Diseases - prevention & control
Recombinant Proteins - biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - isolation & purification
Vaccination - veterinary
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Viral Structural Proteins - biosynthesis
Viral Structural Proteins - genetics
Viral Structural Proteins - immunology
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
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Summary:The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). The entire sequence region encoding VP2, VP4, and VP3 in that order was cloned and sequenced. Following subcloning into the Escherichia coli expression vector pET21a under the T7 promoter, viral proteins were expressed and processed as demonstrated by Western blot analysis. Virus-like particles could be visualized by immuno-electron microscopy in IPTG-induced cells suggesting that viral assembly can take place in E. coli. Induction of anti-IBDV antibodies was detected in chickens immunized with purified recombinant IBDV by intra muscular (i.m.) injection. Furthermore, the vaccinated chickens were protected when challenged with the Gep 5 isolate of IBDV.
ISSN:0920-8569
1572-994X
DOI:10.1023/A:1025780611356