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Vaccination with E. coli Recombinant Empty Viral Particles of Infectious Bursal Disease Virus (IBDV) Confer Protection
The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). The entire sequence region encoding VP2, VP4, and VP3 in that order was cloned and sequenced. Following subcloning into the Escherichia coli expression vector...
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| Published in: | Virus genes 2003-10, Vol.27 (2), p.169-175 |
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| Main Authors: | , , , , , , , , , |
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| container_end_page | 175 |
| container_issue | 2 |
| container_start_page | 169 |
| container_title | Virus genes |
| container_volume | 27 |
| creator | Rogel, Arie Benvenisti, Luna Sela, Ilan Edelbaum, Orit Tanne, Edna Shachar, Yehoshua Zanberg, Yehuda Gontmakher, Tanya Khayat, Eli Stram, Yehuda |
| description | The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). The entire sequence region encoding VP2, VP4, and VP3 in that order was cloned and sequenced. Following subcloning into the Escherichia coli expression vector pET21a under the T7 promoter, viral proteins were expressed and processed as demonstrated by Western blot analysis. Virus-like particles could be visualized by immuno-electron microscopy in IPTG-induced cells suggesting that viral assembly can take place in E. coli. Induction of anti-IBDV antibodies was detected in chickens immunized with purified recombinant IBDV by intra muscular (i.m.) injection. Furthermore, the vaccinated chickens were protected when challenged with the Gep 5 isolate of IBDV. |
| doi_str_mv | 10.1023/A:1025780611356 |
| format | article |
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Furthermore, the vaccinated chickens were protected when challenged with the Gep 5 isolate of IBDV.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1023/A:1025780611356</identifier><identifier>PMID: 14501195</identifier><language>eng</language><publisher>United States: Kluwer Academic Publishers</publisher><subject>Animals ; Antibodies, Viral - biosynthesis ; Birnaviridae Infections - immunology ; Birnaviridae Infections - prevention & control ; Birnaviridae Infections - veterinary ; Blotting, Western ; Chickens - immunology ; Cloning, Molecular ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Genetic Vectors ; Infectious bursal disease virus - genetics ; Infectious bursal disease virus - immunology ; Microscopy, Immunoelectron ; Poultry Diseases - immunology ; Poultry Diseases - prevention & control ; Recombinant Proteins - biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - isolation & purification ; Vaccination - veterinary ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - immunology ; Viral Structural Proteins - biosynthesis ; Viral Structural Proteins - genetics ; Viral Structural Proteins - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology</subject><ispartof>Virus genes, 2003-10, Vol.27 (2), p.169-175</ispartof><rights>Copyright Kluwer Academic Publishers Oct 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14501195$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rogel, Arie</creatorcontrib><creatorcontrib>Benvenisti, Luna</creatorcontrib><creatorcontrib>Sela, Ilan</creatorcontrib><creatorcontrib>Edelbaum, Orit</creatorcontrib><creatorcontrib>Tanne, Edna</creatorcontrib><creatorcontrib>Shachar, Yehoshua</creatorcontrib><creatorcontrib>Zanberg, Yehuda</creatorcontrib><creatorcontrib>Gontmakher, Tanya</creatorcontrib><creatorcontrib>Khayat, Eli</creatorcontrib><creatorcontrib>Stram, Yehuda</creatorcontrib><title>Vaccination with E. coli Recombinant Empty Viral Particles of Infectious Bursal Disease Virus (IBDV) Confer Protection</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><description>The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). 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Furthermore, the vaccinated chickens were protected when challenged with the Gep 5 isolate of IBDV.</description><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Birnaviridae Infections - immunology</subject><subject>Birnaviridae Infections - prevention & control</subject><subject>Birnaviridae Infections - veterinary</subject><subject>Blotting, Western</subject><subject>Chickens - immunology</subject><subject>Cloning, Molecular</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Genetic Vectors</subject><subject>Infectious bursal disease virus - genetics</subject><subject>Infectious bursal disease virus - immunology</subject><subject>Microscopy, Immunoelectron</subject><subject>Poultry Diseases - immunology</subject><subject>Poultry Diseases - prevention & control</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - 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Academic</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rogel, Arie</au><au>Benvenisti, Luna</au><au>Sela, Ilan</au><au>Edelbaum, Orit</au><au>Tanne, Edna</au><au>Shachar, Yehoshua</au><au>Zanberg, Yehuda</au><au>Gontmakher, Tanya</au><au>Khayat, Eli</au><au>Stram, Yehuda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination with E. coli Recombinant Empty Viral Particles of Infectious Bursal Disease Virus (IBDV) Confer Protection</atitle><jtitle>Virus genes</jtitle><addtitle>Virus Genes</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>27</volume><issue>2</issue><spage>169</spage><epage>175</epage><pages>169-175</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>The A genome segment of the highly virulent Infectious bursal disease virus (IBDV) was amplified using long and accurate-RT-PCR (LA-RT-PCR). The entire sequence region encoding VP2, VP4, and VP3 in that order was cloned and sequenced. Following subcloning into the Escherichia coli expression vector pET21a under the T7 promoter, viral proteins were expressed and processed as demonstrated by Western blot analysis. Virus-like particles could be visualized by immuno-electron microscopy in IPTG-induced cells suggesting that viral assembly can take place in E. coli. Induction of anti-IBDV antibodies was detected in chickens immunized with purified recombinant IBDV by intra muscular (i.m.) injection. Furthermore, the vaccinated chickens were protected when challenged with the Gep 5 isolate of IBDV.</abstract><cop>United States</cop><pub>Kluwer Academic Publishers</pub><pmid>14501195</pmid><doi>10.1023/A:1025780611356</doi><tpages>7</tpages></addata></record> |
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| ispartof | Virus genes, 2003-10, Vol.27 (2), p.169-175 |
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| language | eng |
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| source | Springer Nature |
| subjects | Animals Antibodies, Viral - biosynthesis Birnaviridae Infections - immunology Birnaviridae Infections - prevention & control Birnaviridae Infections - veterinary Blotting, Western Chickens - immunology Cloning, Molecular Escherichia coli - genetics Escherichia coli - metabolism Genetic Vectors Infectious bursal disease virus - genetics Infectious bursal disease virus - immunology Microscopy, Immunoelectron Poultry Diseases - immunology Poultry Diseases - prevention & control Recombinant Proteins - biosynthesis Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - isolation & purification Vaccination - veterinary Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology Viral Structural Proteins - biosynthesis Viral Structural Proteins - genetics Viral Structural Proteins - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology |
| title | Vaccination with E. coli Recombinant Empty Viral Particles of Infectious Bursal Disease Virus (IBDV) Confer Protection |
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