Caveolin-1 as tumor suppressor gene in breast cancer

Many articles have reported the caveolin-1 gene to be downregulated thus suggesting that it might be a candidate tumor suppressor gene in many tumors, but in bladder tumors the caveolin-1 gene was expressed and related to the pathological grade. We investigated the function of caveolin-1 protein in...

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Bibliographic Details
Published in:Surgery today (Tokyo, Japan) Japan), 2003-01, Vol.33 (7), p.486-490
Main Authors: Hino, Masato, Doihara, Hiroyoshi, Kobayashi, Kazuyasu, Aoe, Motoi, Shimizu, Nobuyoshi
Format: Article
Language:English
Subjects:
Breast Neoplasms - genetics
Caveolin 1
Caveolins - genetics
Female
Genes, Tumor Suppressor
Humans
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Tumor Cells, Cultured
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Summary:Many articles have reported the caveolin-1 gene to be downregulated thus suggesting that it might be a candidate tumor suppressor gene in many tumors, but in bladder tumors the caveolin-1 gene was expressed and related to the pathological grade. We investigated the function of caveolin-1 protein in breast tumors. We introduced the caveolin-1 gene into a MCF-7 human breast cancer cell line, and examined its cell growth, cell viability, and anchorage-independent growth. The caveolin-1 transfectants showed less proliferation than the vector control transfectants on the fourth day regarding the cell growth rate. In addition, the cell viability of the caveolin-1 transfectants was about 55% of the vector control. A soft agar assay of the caveolin-1 transfectants showed less growth with a lower number of colonies. In MCF-7 cells, the caveolin-1 gene may influence the tumor suppressor efficacy.
ISSN:0941-1291
1436-2813
DOI:10.1007/s10595-002-2538-4