The effect of granulocyte colony-stimulating factor in the treatment of Escherichia coli peritonitis with or without ceftriaxone in a nonneutropenic rat model

• Published in: Surgery today (Tokyo, Japan) Japan), 2003-01, Vol.33 (7), p.504-508
• Main Authors: Saba, Rabin, Güler, Muhsin, Inan, Dilara, Oğünç, Dilara, Atalay, Sakir, Mamikoğlu, Latife, Günseren, Filiz
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - therapeutic use; Ceftriaxone - therapeutic use; Escherichia coli Infections - blood; Escherichia coli Infections - drug therapy; Escherichia coli Infections - mortality; Granulocyte Colony-Stimulating Factor - therapeutic use; Leukocyte Count; Male; Neutrophils; Peritonitis - blood; Peritonitis - drug therapy; Peritonitis - microbiology; Peritonitis - mortality; Rats; Survival Rate
• ISSN: 0941-1291; 1436-2813
• DOI: 10.1007/s10595-002-2539-3

To investigate the effect of granulocyte colony-stimulating factor (G-CSF) in the treatment of Escherichia coli peritonitis with and without ceftriaxone in a nonneutropenic rat model. The rats were divided into five groups: control group (C) receiving physiological saline; peritonitis group (P) infected intraperitoneally with a live bacterial suspension of E. coli; peritonitis and antibiotic group (PA) receiving ceftriaxone 3 h after being infected; peritonitis, antibiotic, and G-CSF group (PAG) receiving G-CSF and antibiotic 3 h after infection; and peritonitis and G-CSF group (PG). All rats in group C survived. Any animals which did not survive died within 24h after inoculation. A significantly higher rate of survival, 95%, was observed with antibiotic treatment alone (PA), in comparison to the G-CSF-treated groups, PAG and PG, 52% and 57%, respectively. No beneficial effect of G-CSF treatment was seen in the E. coli peritonitis and antibiotic therapy remains the basic treatment for this disease.