Immunodominance in the T-cell response to multiple non-H-2 histocompatibility antigens. V: Chromosomal mapping of the immunodominant cytotoxic T-cell target-1 (CTT-1)

• Published in: Immunogenetics (New York) 1993, Vol.38 (2), p.157-160
• Main Authors: VAGLIANI, M, MELANI, C, PARMIANI, G, D'EUSTACHIO, P, WETTSTEIN, P. J, COLOMBO, M. P
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Chromosome Mapping; Fundamental and applied biological sciences. Psychology; Genes, Dominant; Genes. Genome; Genetic Linkage; Histocompatibility Antigens - immunology; Mice; Molecular and cellular biology; Molecular genetics; T-Lymphocytes, Cytotoxic - immunology
• ISSN: 0093-7711; 1432-1211
• DOI: 10.1007/BF00190905

The histocompatibility (H) system comprises a large number of loci responsible for acceptance and rejection of both neoplastic and normal tissue. The H-2 locus is the mouse major histocompatibility complex (MHC). The BALB.B and C57BL/6 mouse strains are both homozygous for the b haplotype of H-2, while the two strains differ in the alleles they carry of many minor histocompatibility determinants. Nevertheless, the cellular immune response generated by C57BL/6 mice challenged with BALB.B splenocytes is dominated by a small number of antigenic determinants. We have named them cytotoxic T-cell target (CTT) antigens. Competition studies suggested that CTT-1 antigenic determinant was shared by BALB.B and C3H.SW mice. The limited genetic data available thus raised the possibility that the CTT-1 phenotype might reflect the action of closely related genes mapping to independently segregating loci in the two inbred strains. To test this possibility directly, we have typed the remaining BXH RI strains, carried out a complementation test to determine the number of loci that contribute to the CTT-1 phenotype of BALB, C3H, and C57BL mice, and have typed backcross progeny to define and map the genetic loci involved.