Dipeptidyl peptidase-IV expression and activity in human glomerular endothelial cells

Glucagon-like peptide-1 (GLP-1), a meal-stimulated gastrointestinal insulinotropic hormone inactivated by dipeptidyl peptidase-IV (DPP-IV), is reduced in type 2 diabetic patients. The present study shows that 2-week exposure of human glomerular endothelial cells to high glucose (22 mM) determines a...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-10, Vol.310 (1), p.28-31
Main Authors: Pala, Laura, Mannucci, Edoardo, Pezzatini, Anna, Ciani, Silvia, Sardi, Jacopo, Raimondi, Laura, Ognibene, Agostino, Cappadona, Angela, Vannelli, Barbara G, Rotella, Carlo M
Format: Article
Language:English
Subjects:
Base Sequence
Buffers
Cells, Cultured
Diabetes mellitus
Dipeptidyl Peptidase 4 - genetics
Dipeptidyl Peptidase 4 - metabolism
DNA Primers
Endothelial cells
Endothelial Cells - enzymology
GLP-1
Glucagon-Like Peptide 1
Glucose
Glucose - administration & dosage
Humans
Kidney Glomerulus - cytology
Kidney Glomerulus - enzymology
Peptidase
Peptides - antagonists & inhibitors
RNA, Messenger - genetics
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Summary:Glucagon-like peptide-1 (GLP-1), a meal-stimulated gastrointestinal insulinotropic hormone inactivated by dipeptidyl peptidase-IV (DPP-IV), is reduced in type 2 diabetic patients. The present study shows that 2-week exposure of human glomerular endothelial cells to high glucose (22 mM) determines a highly significant increase in DPP-IV activity and mRNA expression, which cannot be entirely accounted for by hyperosmolarity. On the other hand, incubation of purified DPP-IV in a buffer solution added with high glucose does not affect enzyme activity. These results suggest that high glucose increases expression and activity of DPP-IV, possibly contributing to GLP-1 reduction in type 2 diabetic patients.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2003.08.111