Polarized Secretion of Tissue-Plasminogen Activator in Cultured Thyroid Cells

We studied the polarized secretion of tissue-type plasminogen activator in porcine thyroid cells cultured as a monolayer on porous bottom chambers. The presence of tissue-type plasminogen activator was detected by zymographic analysis on two independent media that were in contact either with the api...

Full description

Saved in:
Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology - Animal 1993-02, Vol.29A (2), p.161-164
Main Authors: DESRUISSEAU-GONZALVEZ, S, DELORI, P, GRUFFAT, D, CHABAUD, O
Format: Article
Language:English
Subjects:
Animals
Antigens
Biological and medical sciences
Cell lines
Cell physiology
Cells, Cultured
Culture Media
Cultured cells
Enzyme-Linked Immunosorbent Assay
Epithelial Cells
Epithelium - drug effects
Epithelium - enzymology
Fundamental and applied biological sciences. Psychology
Gels
Gene expression regulation
Growth, Differentiation and Senescence
Hepatocytes
Kinetics
Molecular and cellular biology
Plasminogen activators
Secretion
Secretion. Exocytosis
Sertoli cells
Swine
Thyroid Gland - cytology
Thyroid Gland - drug effects
Thyroid Gland - enzymology
Thyrotropin - pharmacology
Tissue Plasminogen Activator - analysis
Tissue Plasminogen Activator - metabolism
Tissue Plasminogen Activator - secretion
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied the polarized secretion of tissue-type plasminogen activator in porcine thyroid cells cultured as a monolayer on porous bottom chambers. The presence of tissue-type plasminogen activator was detected by zymographic analysis on two independent media that were in contact either with the apical surface or with the basolateral membrane. The amount of tissue-type plasminogen activator was determined in both media by ELISA and enzyme assay. Measurable tissue-type plasminogen activator activity was found in the basal but not in the apical medium. However, on zymogram, a lytic zone corresponding to tissue-type plasminogen activator was visible in both media. In addition, a lytic band at 130 kDa suggested presence of a complex formed by tissue-type plasminogen activator and an inhibitor. Preferential basolateral tissue-type plasminogen activator antigen secretion (70%) has been observed, showing the possible relation between tissue-type plasminogen activator and extracellular matrix components. Neither tissue-type plasminogen activator level nor polarized secretion seemed to be regulated by thyrotropin (0.1 mU/ml).
ISSN:0883-8364
2327-431X
1543-706X
DOI:10.1007/BF02630948