EI-1941-1 and -2, Novel Interleukin-1β Converting Enzyme Inhibitors Produced by Farrowia sp. E-1941: II. Taxonomy of Producing Strain, Fermentation, Isolation, Physico-chemical Properties, and Biological Properties

• Published in: Journal of antibiotics 2003/07/25, Vol.56(7), pp.603-609
• Main Authors: KOIZUMI, FUMITO, ISHIGURO, HIROKI, ANDO, KATSUHIKO, KONDO, HIDEMASA, YOSHIDA, MAYUMI, MATSUDA, YUZURU, NAKANISHI, SATOSHI
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Bones, joints and connective tissue. Antiinflammatory agents; Caspase Inhibitors; Chromatography, Gel; Cyclohexanones - chemistry; Cyclohexanones - isolation & purification; Cyclohexanones - pharmacology; Cysteine Proteinase Inhibitors - biosynthesis; Cysteine Proteinase Inhibitors - chemistry; Cysteine Proteinase Inhibitors - isolation & purification; Cysteine Proteinase Inhibitors - pharmacology; Epoxy Compounds - chemistry; Epoxy Compounds - isolation & purification; Epoxy Compounds - pharmacology; Farrowia; Fermentation; Humans; Medical sciences; Microbial Sensitivity Tests; Molecular Structure; Pharmacology. Drug treatments; Serpins - biosynthesis; Serpins - chemistry; Serpins - isolation & purification; Serpins - pharmacology; Soil Microbiology; Sordariales - chemistry; Sordariales - metabolism; Spectrometry, Mass, Fast Atom Bombardment; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.56.603

EI-1941-1 and -2, novel interleukin-1β converting enzyme (ICE) inhibitors, were isolated from the culture broths of Farrowia sp. E-1941. EI-1941-1 and -2 selectively inhibited the human recombinant ICE activity with IC50 values of 0.086 and 0.006μM, respectively. Taxonomy and fermentation of the producing strain and isolation, physico-chemical properties, structure elucidation, and biological properties of EI-1941-1 and -2 are described.