A pentapeptide from type I procollagen promotes extracellular matrix production

The NH2 and COOH propieces of fibril-forming collagens are cleaved off extracellularly and have been implicated in feedback regulation of their own synthesis. Recently, we showed that a subfragment of the carboxyl-terminal propeptide of type I collagen (residues 197-241) dramatically augments extrac...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1993-05, Vol.268 (14), p.9941-9944
Main Authors: Katayama, K., Armendariz-Borunda, J., Raghow, R., Kang, A.H., Seyer, J.M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cell Line
Cells, Cultured
Chick Embryo
Extracellular Matrix - drug effects
Extracellular Matrix - physiology
Fibronectins - biosynthesis
Fundamental and applied biological sciences. Psychology
Glycoproteins
Humans
Kinetics
Molecular Sequence Data
Peptide Fragments - pharmacology
Procollagen - biosynthesis
Procollagen - pharmacology
Proteins
Transforming Growth Factor beta - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The NH2 and COOH propieces of fibril-forming collagens are cleaved off extracellularly and have been implicated in feedback regulation of their own synthesis. Recently, we showed that a subfragment of the carboxyl-terminal propeptide of type I collagen (residues 197-241) dramatically augments extracellular matrix production in subconfluent fibroblasts. This stimulation of type I collagen, type III collagen, and fibronectin production occurred in a dose- and time-dependent manner with no effect on total protein synthesis or on the ratio of secreted proteins to cell-associated proteins (Katayama, K., Seyer, J.M., Raghow, R., and Kang, A.H. (1991) Biochemistry 30, 7097-7104). In the present report, we have extensively dissected this subfragment of the propeptide and found that the pentapeptide Lys-Thr-Thr-Lys-Ser (residues 212-216) is the minimum sequence necessary for potent stimulation of collagen and fibronectin production in a variety of mesenchymal cells. We postulate that the extracellular matrix production in fibroblasts may be subject to either positive or negative feedback regulation depending on the repertoire of specific proteases during postinflammatory tissue regeneration and fibrosis.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)82153-6