Obstructive uropathy in mice and humans: Potential role for PDGF-D in the progression of tubulointerstitial injury

• Published in: Journal of the American Society of Nephrology 2003-10, Vol.14 (10), p.2544-2555
• Main Authors: TANEDA, Sekiko, HUDKINS, Kelly L, TOPOUZIS, Stavros, GILBERTSON, Debra G, OPHASCHAROENSUK, Vuddhidej, TRUONG, Luan, JOHNSON, Richard J, ALPERS, Charles E
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Animals; Biological and medical sciences; Capillaries - metabolism; Cell Division; Collagen Type I - metabolism; Disease Progression; Fibroblasts - metabolism; Fibroblasts - pathology; Fibrosis; Glomerular Mesangium - blood supply; Glomerular Mesangium - metabolism; Glomerular Mesangium - pathology; Humans; Interstitial nephritis; Lymphokines - metabolism; Male; Medical sciences; Mice; Nephritis, Interstitial - metabolism; Nephrology. Urinary tract diseases; Nephropathies. Renovascular diseases. Renal failure; Platelet-Derived Growth Factor - metabolism; Receptor, Platelet-Derived Growth Factor alpha - metabolism; Receptor, Platelet-Derived Growth Factor beta - metabolism; Up-Regulation; Ureteral Obstruction - metabolism; Ureteral Obstruction - pathology
• ISSN: 1046-6673; 1533-3450
• DOI: 10.1097/01.ASN.0000089828.73014.C8

Tubulointerstitial fibrosis is a major characteristic of progressive renal diseases. Platelet-derived growth factor (PDGF) is a family of growth regulatory molecules consisting of PDGF-A and -B, along with the newly discovered PDGF-C and -D. They signal through cell membrane receptors, PDGF receptor alpha (PDGF-Ralpha) and receptor beta (PDGF-Rbeta). Involvement of PDGF-B and PDGF-Rbeta in the initiation and progression of renal fibrosis has been well documented. The authors studied the localization of PDGF ligands and receptors by immunohistochemistry, with emphasis on the role of PDGF-D in murine renal fibrosis induced by unilateral ureteral obstruction (UUO). In mice with UUO, de novo expression of PDGF-D was detected in interstitial cells at day 4, which increased to maximal expression at day 14. Increased expression of PDGF-B by interstitial cells and in some tubules was observed after day 4. The diseased mice did not show augmentation of PDGF-A or PDGF-C proteins in the areas of fibrosis. PDGF-Ralpha and -Rbeta protein expression was increased in interstitial cells after day 4 and reached maximal expression at day 14. Human renal nephrectomies (n = 10) of chronic obstructive nephropathy demonstrated similar de novo expression of PDGF-D in interstitial cells, correlating with expression of PDGF-Rbeta and PDGF-B, as it did in the murine model. These observations suggest that PDGF-D plays an important role in the pathogenesis of tubulointerstitial injury through binding of PDGF-Rbeta in both human obstructive nephropathy and the corresponding murine model of UUO.