Structure of the O-polysaccharide from the lipopolysaccharide of Hafnia alvei strain PCM 1546

An acidic O-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of Hafnia alvei PCM 1546 is composed of d-Gal, d-Glc , d-GlcA, d-GalNAc and O-acetyl groups in the ratios 1:1:1:2:1.6. On the basis of sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectrosco...

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Bibliographic Details
Published in:Carbohydrate research 2003-09, Vol.338 (20), p.2153-2158
Main Authors: Katzenellenbogen, Ewa, Kocharova, Nina A., Zatonsky, George V., Korzeniowska-Kowal, Agnieszka, Shashkov, Alexander S., Knirel, Yuriy A.
Format: Article
Language:English
Subjects:
Bacterial polysaccharide structures
Carbohydrate Sequence
Hafnia alvei
Hafnia alvei - chemistry
Hydrolysis
Lipopolysaccharide
Lipopolysaccharides - chemistry
Lipopolysaccharides - isolation & purification
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens - chemistry
O-Antigen
Polysaccharides, Bacterial - chemistry
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Summary:An acidic O-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of Hafnia alvei PCM 1546 is composed of d-Gal, d-Glc , d-GlcA, d-GalNAc and O-acetyl groups in the ratios 1:1:1:2:1.6. On the basis of sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectroscopy, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: Graphic
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(03)00338-0