Marinimicrobium haloxylanilyticum sp. nov., a new moderately halophilic, polysaccharide-degrading bacterium isolated from Great Salt Lake, Utah

• Published in: Antonie van Leeuwenhoek 2010-11, Vol.98 (4), p.553-565
• Main Authors: Møller, Mette Fogh, Kjeldsen, Kasper Urup, Ingvorsen, Kjeld
• Format: Article
• Language: English
• Subjects: Alteromonadaceae - classification; Alteromonadaceae - cytology; Alteromonadaceae - isolation & purification; Alteromonadaceae - physiology; Animal, plant and microbial ecology; Bacteria; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Biomedical and Life Sciences; Carbohydrates; Carboxymethylcellulose; Cellulose; DNA; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Drug Resistance, Bacterial; Enzymes; Fatty acids; Fundamental and applied biological sciences. Psychology; gamma-Proteobacteria; Genes, rRNA; Geologic Sediments - microbiology; Halophile; Hypersaline; Lakes; Life Sciences; Medical Microbiology; Microbial ecology; Microbiology; Molecular Sequence Data; New species; Original Paper; pH effects; Phenotype; Phylogeny; Plant Sciences; Polymers; RNA, Ribosomal, 16S - analysis; rRNA 16S; Salinity; Salinity effects; Salt lakes; Salt-Tolerance; Sediments; Sodium chloride; Sodium Chloride - metabolism; Soil Science & Conservation; Starch; Systematics; Taxonomy; Temperature effects; Utah; Various environments (extraatmospheric space, air, water); Xylan; Xylan degradation
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-010-9472-y

A new moderately halophilic, strictly aerobic, Gram-negative bacterium, strain SX15T, was isolated from hypersaline surface sediment of the southern arm of Great Salt Lake (Utah, USA). The strain grew on a number of carbohydrates and carbohydrate polymers such as xylan, starch, carboxymethyl cellulose and galactomannan. The strain grew at salinities ranging from 2 to 22% NaCl (w/v). Optimal growth occurred in the presence of 7-11% NaCl (w/v) at a temperature of 35°C and a pH of 6.7-8.2. Major whole-cell fatty acids were C16:0 (30.5%), C18:0 (14.8%), C18:1ω7c (13.1%) and C12:0 (7.8%). The G+C content of the DNA was 60 ± 0.5 mol%. By 16S rRNA gene sequence analysis, strain SX15T was shown to be affiliated to members of the gammaproteobacterial genus Marinimicrobium with pair wise identity values of 92.9-94.6%. The pheno- and genotypic properties suggest that strain SX15T represents a novel species of the genus Marinimicrobium for which the name Marinimicrobium haloxylanilyticum is proposed. The type strain is SX15T (= DSM 23100T = CCUG 59572T).