Suppressive effects of genistein on oxidative stress and NFkappaB activation in RAW 264.7 macrophages

This study was designed to investigate whether genistein may ameliorate oxidative stress and nuclear factor kappaB (NFkappaB) activation in the lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with genistein significantly reduced lipopolysacchar...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-09, Vol.67 (9), p.1916-1922
Main Authors: Choi, Chunyeon, Cho, Hyeyeon, Park, Jiyoung, Cho, Chungwon, Song, Youngsun
Format: Article
Language:English
Subjects:
Animals
Catalase - metabolism
Cell Line
Cell Nucleus - metabolism
Genistein - pharmacology
Glutathione - metabolism
Lipopolysaccharides - pharmacology
Macrophages - drug effects
Macrophages - metabolism
Mice
NF-kappa B - metabolism
Nitric Oxide - antagonists & inhibitors
Nitric Oxide - biosynthesis
Oxidative Stress - drug effects
Oxidative Stress - physiology
Superoxide Dismutase - metabolism
Thiobarbituric Acid Reactive Substances - metabolism
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Summary:This study was designed to investigate whether genistein may ameliorate oxidative stress and nuclear factor kappaB (NFkappaB) activation in the lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with genistein significantly reduced lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in a dose-dependent manner with an IC50 of 69.4 microM. Genistein at 50 microM and 100 microM concentrations reduced thiobarbituric acid-reactive substances (TBARS) accumulation, increasing the GSH level and antioxidant enzyme activities, such as superoxide dismutase (SOD) and catalase. The specific DNA-binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from 50 microM and 100 microM genistein treatments were significantly suppressed. These results suggest that genistein has mild antioxidant activity to suppress intracellular oxidative stress and NFkappaB activation.
ISSN:0916-8451