Determination of Platinum in Urine, Ultrafiltrate, and Whole Plasma by Isotope Dilution Gas Chromatography-Mass Spectrometry Compared to Electrothermal Atomic Absorption Spectrometry

Isotope dilution gas chromatography-mass spectrometry (GC-MS) and electrothermal atomic absorption spectrometry (EAAS) are compared for platinum (Pt) determination in urine, plasma ultrafiltrate, and plasma samples from a patient undergoing cisplatin therapy. The isotope dilution GC-MS method is bas...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 1993-04, Vol.210 (1), p.113-118
Main Authors: Aggarwal, S.K., Gemma, N.W., Kinter, M., Nicholson, J., Shipe, J.R., Herold, D.A.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Carcinoma, Squamous Cell - drug therapy
Carcinoma, Squamous Cell - metabolism
Cisplatin - pharmacokinetics
Cisplatin - therapeutic use
Evaluation Studies as Topic
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Humans
Laryngeal Neoplasms - drug therapy
Laryngeal Neoplasms - metabolism
Male
Medical sciences
Middle Aged
Pharmacology. Drug treatments
Platinum - blood
Platinum - urine
Spectrophotometry, Atomic - methods
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Isotope dilution gas chromatography-mass spectrometry (GC-MS) and electrothermal atomic absorption spectrometry (EAAS) are compared for platinum (Pt) determination in urine, plasma ultrafiltrate, and plasma samples from a patient undergoing cisplatin therapy. The isotope dilution GC-MS method is based on the use of lithium bis(trifluoroethyl)dithiocarbamate as a chelating agent and enriched 192Pt as an internal standard. Pt isotope ratios were measured using a Finnigan MAT 8230 organic mass spectrometer, and Pt concentrations were calculated from different sets of isotope ratios in the molecular ion of the Pt-chelate. In the EAAS method, Pt concentrations were determined using three different approaches. These were (i) calibration curve based on aqueous standards containing Pt in 10% HCl, (ii) standard addition, and (iii) matrix digestion followed by standard addition. Good agreement was obtained for Pt concentrations determined by GC-MS and EAAS in urine samples while there were significant differences in Pt concentrations of ultrafiltrate and whole plasma samples by the two methods. Discussion of possible reasons for these differences emphasizes the need for future critical evaluation of these methods.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1993.1159