The Adenomatous Polyposis Coli (APC) gene microsatellite marker D5S1385 is equally informative for loss of heterozygosity as the marker D5S346

Molecular analyses for loss of heterozygosity (LOH) at a gene locus are used to identify genomic imbalance in tumor tissue. A frequently utilized microsatellite marker for the Adenomatous Polyposis Coli (APC) gene is denoted D5S346. However, when an individual has two identical forms of this microsa...

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Bibliographic Details
Published in:Experimental and molecular pathology 2003-10, Vol.75 (2), p.144-147
Main Authors: Zauber, N.Peter, Sabbath-Solitare, Marlene, Marotta, Stephen P, Bishop, D.Timothy
Format: Article
Language:English
Subjects:
Adenomatous Polyposis Coli - genetics
Adenomatous Polyposis Coli gene
Adult
Aged
Aged, 80 and over
Alleles
Biological and medical sciences
Colon carcinoma
D5S1385
D5S346
DNA Primers - chemistry
DNA, Neoplasm - analysis
Female
Gastroenterology. Liver. Pancreas. Abdomen
Genes, APC
Genotype
Homozygosity
Humans
Loss of heterozygosity
Loss of Heterozygosity - genetics
Male
Medical sciences
Microsatellite markers
Microsatellite Repeats - genetics
Middle Aged
Mutation - genetics
Polymerase Chain Reaction
Polymorphism, Genetic
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:Molecular analyses for loss of heterozygosity (LOH) at a gene locus are used to identify genomic imbalance in tumor tissue. A frequently utilized microsatellite marker for the Adenomatous Polyposis Coli (APC) gene is denoted D5S346. However, when an individual has two identical forms of this microsatellite, then a second microsatellite, also near the APC gene, is needed. We present data on an APC microsatellite marker designated D5S1385, located on the 5′ end of the APC gene, and we compare these to data utilizing the marker D5S346. Polymerase chain reaction (PCR) was used, followed by gel electrophoresis. Homozygosity for the marker D5S1385 was present in 55 individuals, or 28%. Thirty-seven carcinomas and 32 adenomas were assessed for LOH from individuals informative with both microsatellite markers. D5S1385 detected LOH in 39 of 41 lesions, or 95%, for which D5S346 detected LOH. D5S1385 is moderately polymorphic and is informative at least as often as D5S346.
ISSN:0014-4800
1096-0945
DOI:10.1016/S0014-4800(03)00069-8