Identification of Aeromonas schubertii and Aeromonas jandaei by using a polymerase chain reaction‐probe test

Two oligonucleotide primers were used in a polymerase chain reaction‐protocol to amplify a region (approx. 850 bp) of the 16S rRNA gene of Aeromonas schubertii and Aeromonas jandaei. Hybridization of the polymerase chain reaction products to specific internal probes provided a highly specific method...

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Bibliographic Details
Published in:FEMS microbiology letters 1993-04, Vol.108 (2), p.151-155
Main Authors: Ash, Carol, Martinez‐Murcia, A.J., Collins, M.D.
Format: Article
Language:English
Subjects:
16S rRNA gene probe
Aeromonas
Aeromonas - classification
Aeromonas - genetics
Aeromonas jandaei
Aeromonas schubertii
Base Sequence
Blotting, Southern
DNA, Ribosomal - genetics
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction
RNA, Ribosomal, 16S - genetics
Species Specificity
Taxonomy
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Summary:Two oligonucleotide primers were used in a polymerase chain reaction‐protocol to amplify a region (approx. 850 bp) of the 16S rRNA gene of Aeromonas schubertii and Aeromonas jandaei. Hybridization of the polymerase chain reaction products to specific internal probes provided a highly specific method for the identification of these two species.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1993.tb06091.x