Expression analysis of the mixed function oxidase system in rat brain by the polymerase chain reaction

Metabolism of therapeutic drugs in the body by the mixed function oxidase system is an important consideration in the analysis of a drug's effectiveness. P450-dependent metabolism within the brain of a neuro-specific drug may affect the drug's course of action. To determine whether cytochr...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 1993-03, Vol.120 (2), p.171-179
Main Authors: HODGSON, A. V, WHITE, T. B, WHITE, J. W, STROBEL, H. W
Format: Article
Language:English
Subjects:
Amitriptyline - pharmacology
Analytical, structural and metabolic biochemistry
Animals
Base Sequence
Benzoflavones - pharmacology
beta-Naphthoflavone
Biological and medical sciences
Brain - drug effects
Brain - enzymology
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P-450 Enzyme System - genetics
Enzyme Induction - drug effects
Enzymes and enzyme inhibitors
Female
Fundamental and applied biological sciences. Psychology
Imipramine - pharmacology
Isoenzymes - biosynthesis
Isoenzymes - genetics
Male
Mixed Function Oxygenases - biosynthesis
Mixed Function Oxygenases - genetics
Molecular Sequence Data
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - genetics
Oxidoreductases
Phenobarbital - pharmacology
Polymerase Chain Reaction
Rats
Rats, Sprague-Dawley - metabolism
RNA, Messenger - biosynthesis
Safrole - pharmacology
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Summary:Metabolism of therapeutic drugs in the body by the mixed function oxidase system is an important consideration in the analysis of a drug's effectiveness. P450-dependent metabolism within the brain of a neuro-specific drug may affect the drug's course of action. To determine whether cytochrome P450 was expressed in brain, RNA was isolated from the whole brains of rats treated with a variety of known hepatic P450 inducers, including amitriptyline, imipramine, isosafrole, phenobarbital, and beta-naphthoflavone. The RNA was analyzed for the presence of P450 isozymes by the PCR technique. Differential expression of P450IA1, P450IIB1, P450IIB2, P450IID, and P450IIE1 was detected in the brain samples, depending on the treatment. Cytochrome P450 reductase expression was also detected in the brain samples, giving strong evidence that the brain contains a competent mixed function oxidase system under all conditions studied.
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00926090