Agrobacterium-tumefaciens-mediated transformation of Helminthosporium turcicum, the maize leaf-blight fungus

Agrobacterium tumefaciens has the ability to transfer its T-DNA to plants, yeast, filamentous fungi, and human cells and integrate it into their genome. Conidia of the maize pathogen Helminthosporium turcicum were transformed to hygromycin B resistance by a Agrobacterium- tumefaciens-mediated transf...

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Bibliographic Details
Published in:Archives of microbiology 2003-10, Vol.180 (4), p.279-284
Main Authors: DEGEFU, Yeshitila, HANIF, Mubashir
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens - genetics
Agrobacterium tumefaciens - metabolism
Bacteria
Bacteriology
Biological and medical sciences
Blotting, Southern
Corn
Deoxyribonucleic acid
DNA
DNA, Bacterial
Fundamental and applied biological sciences. Psychology
Genetics
Helminthosporium - genetics
Hygromycin B - pharmacology
Microbiology
Mycology
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Pathogens
Plasmids - genetics
Proteins
Transformation, Genetic
Yeasts
Zea mays - microbiology
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Summary:Agrobacterium tumefaciens has the ability to transfer its T-DNA to plants, yeast, filamentous fungi, and human cells and integrate it into their genome. Conidia of the maize pathogen Helminthosporium turcicum were transformed to hygromycin B resistance by a Agrobacterium- tumefaciens-mediated transformation system using a binary plasmid vector containing the hygromycin B phosphotransferase ( hph) and the enhanced green fluorescent protein ( EGFP) genes controlled by the gpd promoter from Agaricus bisporus and the CaMV 35S terminator. Agrobacterium- tumefaciens-mediated transformation yielded stable transformants capable of growing on increased concentrations of hygromycin B. The presence of hph in the transformants was confirmed by PCR, and integration of the T-DNA at random sites in the genome was demonstrated by Southern blot analysis. Agrobacterium- tumefaciens-mediated transformation of Helminthosporium turcicum provides an opportunity for advancing studies of the molecular genetics of the fungus and of the molecular basis of its pathogenicity on maize.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-003-0589-5