Size and Stability of Reconstituted Sesame Oil Bodies

Oil bodies of sesame seeds comprise a triacylglycerol matrix, which is surrounded by a monolayer of phospholipids embedded with unique proteins, mainly structural proteins termed oleosins. Artificial oil bodies were successfully reconstituted with various compositions of triacylglycerols, phospholip...

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Bibliographic Details
Published in:Biotechnology progress 2003, Vol.19 (5), p.1623-1626
Main Authors: Peng, Chi-Chung, Lin, I-Ping, Lin, Ching-Kuan, Tzen, Jason T. C.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
genipin
glutaraldehyde
Hydrogen-Ion Concentration
Inclusion Bodies - metabolism
Inclusion Bodies - ultrastructure
oleosins
proteinase K
Seeds - cytology
Seeds - metabolism
sesame oil
Sesame Oil - chemistry
Sesame Oil - isolation & purification
Sesame Oil - metabolism
Sesamum - cytology
Sesamum - metabolism
Sesamum indicum
Surface Properties
Temperature
Triglycerides
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Summary:Oil bodies of sesame seeds comprise a triacylglycerol matrix, which is surrounded by a monolayer of phospholipids embedded with unique proteins, mainly structural proteins termed oleosins. Artificial oil bodies were successfully reconstituted with various compositions of triacylglycerols, phospholipids, and oil‐body proteins. The sizes of reconstituted oil bodies displayed a normal distribution with an average size proportional to the ratio of triacylglycerols to oil‐body proteins. Both thermostability and structural stability of reconstituted oil bodies decreased as their sizes increased, and vice versa. Proteinase K digestion indicated that oleosins anchored both native and reconstituted oil bodies via their central hydrophobic domains. The stability of reconstituted oil bodies, as well as the purified ones from sesame seeds, could be substantially enhanced after their surface proteins were cross‐linked by glutaraldehyde or genipin.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp034129z