A simplified cytomegalovirus pp65 antigenemia assay procedure

A simplified cytomegalovirus (CMV) pp65 antigenemia assay using a one-step erythrocyte lysis, fixation and permeabilization process was compared with a standard protocol, the CMV CINAkit (Argene Biosoft) assay. The results were comparable, both quantitatively and qualitatively. The new method saves...

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Bibliographic Details
Published in:Journal of clinical virology 2003-12, Vol.28 (3), p.317-322
Main Authors: Gratacap-Cavallier, B., Bonadona, A., Berthier, R., Brambilla, E., Seigneurin, J.M., Lorimier, P.
Format: Article
Language:English
Subjects:
Assay
Cytomegalovirus
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - diagnosis
Cytomegalovirus Infections - virology
Fluorescent Antibody Technique
Humans
Leukocytes - virology
Phosphoproteins - blood
pp65 Antigenemia
pp65 protein
Rapid
Reagent Kits, Diagnostic
Time Factors
Viral Matrix Proteins - blood
Viremia - diagnosis
Viremia - virology
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Summary:A simplified cytomegalovirus (CMV) pp65 antigenemia assay using a one-step erythrocyte lysis, fixation and permeabilization process was compared with a standard protocol, the CMV CINAkit (Argene Biosoft) assay. The results were comparable, both quantitatively and qualitatively. The new method saves time. It also provides flexibility because the cell suspension can be stored so that test completion can be deferred if so desired.
ISSN:1386-6532
1873-5967
DOI:10.1016/S1386-6532(03)00075-1