Increase of hybridoma productivity using an original dialysis culture system

Hybridoma cell growth and monoclonal antibody production were investigated with a laboratory-made system in which cells were grown in dialysis tubing (MW cut-off 25 kD). The dialysis system contained 10 ml of cell suspension and was immersed in 200 ml of culture medium which when replaced or was at...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 1993-01, Vol.11 (1), p.41-48
Main Authors: MATHIOT, B, PERANI, A, DUMAS, D, MAUGRAS, M, DIDELON, J, STOLTZ, J.-F
Format: Article
Language:English
Subjects:
Animal cells
Animals
Antibodies, Monoclonal - biosynthesis
Biological and medical sciences
Biotechnology
Cell Division - physiology
Cell hybridization, cell fusion, hybridomas
Cell Survival - physiology
Cells, Cultured
Cost Control
Cytological Techniques
Dialysis - instrumentation
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Humans
Hybridomas - cytology
Hybridomas - metabolism
Methods. Procedures. Technologies
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Summary:Hybridoma cell growth and monoclonal antibody production were investigated with a laboratory-made system in which cells were grown in dialysis tubing (MW cut-off 25 kD). The dialysis system contained 10 ml of cell suspension and was immersed in 200 ml of culture medium which when replaced or was at 4-day intervals. With this system, monoclonal antibody concentrations similar to those observed in ascites (concentrations in the order of one gram per liter) were obtained. With no medium replacement, the antibody production was 3.3 g/l and the cell productivity 3.2 x 10(-8) micrograms of IgM produced per cell in one minute. With medium replacement the antibody production was higher, 4.4 g/l but the cell productivity was lower, 1.49 x 10(-8) micrograms per cell in one minute. Cells cultivated in non-optimized conditions were better producers than cells growing in a good environment.
ISSN:0920-9069
1573-0778
DOI:10.1007/BF00749056