Comparative immunohistolocalization of carbonic anhydrase isozymes I, II and III in the equine and bovine digestive tract

Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in equine and bovine digestive tracts were studied. In the horse, epithelial cells in both the oesophagus and non-glandular part of the stomach lacked all three isozymes. In contrast, surface epithelial and par...

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Bibliographic Details
Published in:The Histochemical journal 1993-04, Vol.25 (4), p.304-311, Article 304
Main Authors: SASAKI, K, IGARASHI, S.-I, AMASAKI, T, AMASAKI, H, NISHITA, T, KANO, Y, ASARI, M
Format: Article
Language:English
Subjects:
Animals
Antibody Specificity
Biological and medical sciences
Carbonic Anhydrases - analysis
Cattle - metabolism
Digestive System - enzymology
Electrophoresis, Polyacrylamide Gel
Female
Fundamental and applied biological sciences. Psychology
Horses - metabolism
Immunoenzyme Techniques
Isoenzymes - analysis
Stomach
Vertebrates: digestive system
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Summary:Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in equine and bovine digestive tracts were studied. In the horse, epithelial cells in both the oesophagus and non-glandular part of the stomach lacked all three isozymes. In contrast, surface epithelial and parietal cells in the glandular region of the stomach showed reactivity for CA-II. In the small intestine, absorptive columnar cells covering the villi in the duodenum were positive for CA-II. The epithelium of the jejunum and ileum lacked all three isozymes. In the large intestine, CA-II was detected in the columnar cells in the upper part of the crypt. In cattle, epithelial cells of the oesophagus showed reactions for CA-I and CA-III but not for CA-II. Although the absorptive epithelial cells of the small intestine lacked CA-I, CA-II and CA-III, those of the upper part of large intestine crypts were heavily stained for all three isozymes.
ISSN:0018-2214
1573-6865
DOI:10.1007/bf00159122