Monoclonal antibodies demonstrate accessible HA2 epitopes in minor subpopulation of native influenza virus haemagglutinin molecules

Haemagglutinin (HA) was detected on the surface of influenza virus infected cell with monoclonal antibodies (MAbs) against both HA glycopolypeptides, HA1 and HA2, however, the reactivity of HA2-specific MAbs was remarkably lower as compared with HA1-specific MAbs. Quantitative analysis with two MAbs...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 1993, Vol.130 (1-2), p.45-56
Main Authors: VARECKOVA, E, MUCHA, V, CIAMPOR, F, BETAKOVA, T, RUSS, G
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibody Specificity
Antigenic determinants, haptens, artificial antigens
Antigens
Binding, Competitive
Biological and medical sciences
Cell Line
Cells, Cultured
Chick Embryo
Dogs
Epitopes - analysis
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hemagglutinin Glycoproteins, Influenza Virus
Hemagglutinins, Viral - analysis
Hemagglutinins, Viral - immunology
Influenza A virus - immunology
Influenza A virus - ultrastructure
influenza virus
Kidney
Kinetics
Microscopy, Electron
Microscopy, Immunoelectron
Molecular immunology
Radioimmunoassay
Viral Envelope Proteins - analysis
Viral Envelope Proteins - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Haemagglutinin (HA) was detected on the surface of influenza virus infected cell with monoclonal antibodies (MAbs) against both HA glycopolypeptides, HA1 and HA2, however, the reactivity of HA2-specific MAbs was remarkably lower as compared with HA1-specific MAbs. Quantitative analysis with two MAbs, IB8 (anti-HA1) and IIF4 (anti-HA2) respectively, revealed that HA2 epitope was reachable for antibody only in minor subpopulation of the HA representing approximately 7% of all molecules (spikes). The basis of the HA heterogeneity is discussed.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01318995