Distribution and proliferation of bone marrow cells in the brain after pilocarpine‐induced status epilepticus in mice

Summary The distribution of bone marrow cells in brain areas during the acute period after pilocarpine‐induced status epilepticus (SE) was investigated here. To achieve this, we generated chimeric mice by engrafting bone marrow cells from enhanced green fluorescent protein (eGFP) transgenic mice. GF...

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Bibliographic Details
Published in:Epilepsia (Copenhagen) 2010-08, Vol.51 (8), p.1628-1632
Main Authors: Longo, Beatriz, Romariz, Simone, Blanco, Miriam Marcela, Vasconcelos, Juliana Fraga, Bahia, Luciana, Soares, Milena Botelho Pereira, Mello, Luiz E., Ribeiro‐dos‐Santos, Ricardo
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bone Marrow Cells - physiology
Bone Marrow Transplantation - methods
Brain - pathology
Brain - surgery
Bromodeoxyuridine - metabolism
Cell Count - methods
Cell Proliferation
Chimeric mice
Disease Models, Animal
GFP
Green Fluorescent Proteins - genetics
Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy
Hippocampus
Male
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microglia
Nerve Tissue Proteins - metabolism
Nervous system (semeiology, syndromes)
Neurology
Neuropharmacology
Neuroprotective agent
Pharmacology. Drug treatments
Pilocarpine - adverse effects
Status epilepticus
Status Epilepticus - chemically induced
Status Epilepticus - pathology
Status Epilepticus - surgery
Time Factors
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Summary:Summary The distribution of bone marrow cells in brain areas during the acute period after pilocarpine‐induced status epilepticus (SE) was investigated here. To achieve this, we generated chimeric mice by engrafting bone marrow cells from enhanced green fluorescent protein (eGFP) transgenic mice. GFP+ bone marrow–derived cells were found throughout the brain, predominantly in the hippocampus. As expected, these cells exhibited the characteristics of microglia. The pattern of distribution, proliferation, and differentiation of GFP+ cells changes as a function of intensity and time following SE. This pattern is also a consequence of the inflammatory response, which is followed by the progressive neuronal damage that is characteristic of the pilocarpine model.
ISSN:0013-9580
1528-1167
DOI:10.1111/j.1528-1167.2010.02570.x