Loss of Muscarinic and Purinergic Receptors in Urinary Bladder of Rats With Hydrochloric Acid-induced Cystitis

Objectives To clarify the basic mechanism involved in the pathophysiology of cystitis by characterizing the urodynamic parameters, pharmacologically relevant (muscarinic and purinergic) receptors, and the in vivo release of adenosine triphosphate (ATP) in the bladder of hydrochloric acid (HCl)-treat...

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Bibliographic Details
Published in:Urology (Ridgewood, N.J.) N.J.), 2010-10, Vol.76 (4), p.1017.e7-1017.e12
Main Authors: Yoshida, Akira, Kageyama, Aiko, Fujino, Tomomi, Nozawa, Yoshihisa, Yamada, Shizuo
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - analogs & derivatives
Adenosine Triphosphate - secretion
Animals
Benzhydryl Compounds - metabolism
Benzofurans - metabolism
Cresols - metabolism
Cystitis - chemically induced
Cystitis - metabolism
Disease Models, Animal
Down-Regulation
Female
Hydrochloric Acid - toxicity
Mandelic Acids - metabolism
N-Methylscopolamine
Organophosphonates - metabolism
Phenols - metabolism
Phenylpropanolamine - metabolism
Pirenzepine - analogs & derivatives
Pirenzepine - metabolism
Polycyclic Compounds - metabolism
Pyridoxal Phosphate - analogs & derivatives
Pyridoxal Phosphate - metabolism
Pyrrolidines - metabolism
Quinuclidines - metabolism
Radioligand Assay
Rats
Rats, Sprague-Dawley
Receptors, Muscarinic - analysis
Receptors, Purinergic - analysis
Solifenacin Succinate
Tetrahydroisoquinolines - metabolism
Tolterodine Tartrate
Urinary Bladder - chemistry
Urinary Bladder - drug effects
Urination
Urodynamics
Urology
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Summary:Objectives To clarify the basic mechanism involved in the pathophysiology of cystitis by characterizing the urodynamic parameters, pharmacologically relevant (muscarinic and purinergic) receptors, and the in vivo release of adenosine triphosphate (ATP) in the bladder of hydrochloric acid (HCl)-treated rats. Methods The muscarinic and purinergic receptors in rat tissue were measured by radioreceptor assays using (N-methyl-3 H) scopolamine methyl chloride ([3 H]NMS) and αβ-methylene-ATP (2,8-3 H) tetrasodium salt ([3 H]αβ-MeATP), respectively. The urodynamic parameters and ATP levels were measured using a cystometric method and the luciferin-luciferase assay, respectively. Results In the HCl-treated rats, the micturition interval and micturition volume were significantly (48% and 55%, respectively, P
ISSN:0090-4295
1527-9995
DOI:10.1016/j.urology.2010.05.012