The Human Kell Blood Group Gene Maps to Chromosome 7q33 and its Expression Is Restricted to Erythroid Cells

The Kell blood group is one of the major antigenic systems in human red blood cells. To determine the location of the Kell gene on human chromosomes, panels containing genomic DNA of human-hamster somatic cell hybrids were hybridized with radiolabeled cDNA probe specific for the Kell locus. Only the...

Full description

Saved in:
Bibliographic Details
Published in:Blood 1993-05, Vol.81 (10), p.2804-2809
Main Authors: Lee, Soohee, Zambas, Eleni D., Marsh, W. Laurence, Redman, Colvin M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Northern
Blotting, Southern
Bone Marrow - physiology
Chromosome Banding
Chromosome Mapping
Chromosomes, Human, Pair 7
Cricetinae
Erythrocytes - physiology
Fetus
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hematopoietic Stem Cells - physiology
Humans
Hybrid Cells
Immunohematology
In Situ Hybridization
Karyotyping
Kell Blood-Group System - genetics
Leukocytes - physiology
Organ Specificity
Phenotype
Poly A - genetics
Poly A - isolation & purification
Red blood cell immunology
RNA - genetics
RNA - isolation & purification
RNA, Messenger
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Kell blood group is one of the major antigenic systems in human red blood cells. To determine the location of the Kell gene on human chromosomes, panels containing genomic DNA of human-hamster somatic cell hybrids were hybridized with radiolabeled cDNA probe specific for the Kell locus. Only the samples containing DNA from chromosome 7 gave positive hybridization signals. In situ hybridization analysis, using genomic clones isolated with the cDNA, localized the KEL gene to 7q33. Northern blot analysis of poly(A)+ RNA from human brain, kidney, lung, fetal and adult liver, and bone marrow showed that Kell transcripts were only present in fetal liver and bone marrow. This indicates that the Kell protein, which carries the Kell antigens, may only be expressed in erythroid tissues.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V81.10.2804.2804