Characterization of insulin-like growth factor 1 in human primary brain tumors

Insulin-like growth factor 1 (IGF-1) is involved in the regulation of brain development and has been suggested as an autocrine stimulator of brain tumor cell proliferation. This study demonstrates the expression of IGF-1 in tumor tissue from human gliomas and one esthesioneuroblastoma. Using immunoh...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1993-06, Vol.53 (11), p.2475-2478
Main Authors: SANDBERG-NORDQVIST, A.-C, STÅHLBOM, P.-A, REINECKE, M, COLLINS, V. P, VON HOLST, H, SARA, V
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
brain
Brain Neoplasms - chemistry
expression
Glioma - chemistry
Humans
insulin-like growth factor 1
Insulin-Like Growth Factor I - analysis
Insulin-Like Growth Factor I - chemistry
man
Medical sciences
Molecular Sequence Data
mRNA
Neuroectodermal Tumors, Primitive, Peripheral - chemistry
Neurology
RNA, Messenger - analysis
RNA, Messenger - chemistry
RNA, Neoplasm - analysis
RNA, Neoplasm - chemistry
splicing
Tumors of the nervous system. Phacomatoses
tumours
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Summary:Insulin-like growth factor 1 (IGF-1) is involved in the regulation of brain development and has been suggested as an autocrine stimulator of brain tumor cell proliferation. This study demonstrates the expression of IGF-1 in tumor tissue from human gliomas and one esthesioneuroblastoma. Using immunohistochemistry, expression of an IGF-1-like peptide was localized in tumor cells of 6 of the 9 gliomas examined as well as the esthesioneuroblastoma. From one anaplastic oligodendroglioma (which showed strong IGF-1 immunostaining) the IGF-1 transcripts were characterized after isolation of mRNA followed by amplification using the reverse transcriptase-polymerase chain reaction. Two IGF-1 complementary DNAs resulting from alternative splicing of the IGF-1 primary transcript were identified. These transcripts encode two different precursor proteins which correspond to Ea IGF-1 and Eb IGF-1. The significance of IGF-1 alternative mRNA splicing pathways remains to be determined.
ISSN:0008-5472
1538-7445