Use of isopropyl alcohol in histological assays: dehydration of tissue, enbessing into paraffin, and processing of paraffin sections

Isopropyl alcohol (isopropanol) can be used as a substitute for ethyl alcohol in tissue dehydration during embedding into paraffin and dehydration of stained sections. The use of isopropyl alcohol during paraffinization allows us to exclude treatment with intermediate solvents of paraffin (chlorofor...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2003-07, Vol.136 (1), p.105-106
Main Authors: Viktorov, I V, Proshin, S S
Format: Article
Language:English
Subjects:
2-Propanol - pharmacology
Benzene - chemistry
Chloroform - chemistry
Fixatives - pharmacology
Histological Techniques
Histology
Paraffin - chemistry
Paraffin Embedding
Staining and Labeling
Xylenes - chemistry
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Summary:Isopropyl alcohol (isopropanol) can be used as a substitute for ethyl alcohol in tissue dehydration during embedding into paraffin and dehydration of stained sections. The use of isopropyl alcohol during paraffinization allows us to exclude treatment with intermediate solvents of paraffin (chloroform, xylene, and benzene), which reduces the degree of tissue compaction and simplifies and accelerates histological assay.
ISSN:0007-4888
1573-8221
DOI:10.1023/A:1026017719668