Molecular cloning and expression of a MAP kinase homologue from pea

The cdc2 kinases are important cell cycle regulators in all eukaryotes. MAP kinases, a closely related family of protein kinases, are involved in cell cycle regulation in yeasts and vertebrates, but previously have not been documented in plants. We used PCR to amplify Brassica napus DNA sequences us...

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Bibliographic Details
Published in:Plant molecular biology 1993-04, Vol.22 (1), p.83-90
Main Authors: Stafstrom, J.P. (Northern Illinois Univ., DeKalb, IL (USA). Dept. of Biological Sciences), Altschuler, M, Anderson, D.H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
BOURGEON
BUDS
Calcium-Calmodulin-Dependent Protein Kinases
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
ETAPAS DE DESARROLLO DE LA PLANTA
EXPRESION GENICA
EXPRESSION DES GENES
Fabaceae - genetics
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Gene Library
Molecular and cellular biology
MOLECULAR CLONING
Molecular genetics
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
PISUM SATIVUM
PLANT DEVELOPMENTAL STAGES
Plants, Medicinal
PROTEIN KINASE
Protein Kinases - biosynthesis
Protein Kinases - genetics
PROTEINA QUINASA
PROTEINE KINASE
Recombinant Fusion Proteins - biosynthesis
RNA, Messenger - analysis
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
STADE DE DEVELOPPEMENT VEGETAL
Tissue Distribution
YEMA (PLANTA)
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Summary:The cdc2 kinases are important cell cycle regulators in all eukaryotes. MAP kinases, a closely related family of protein kinases, are involved in cell cycle regulation in yeasts and vertebrates, but previously have not been documented in plants. We used PCR to amplify Brassica napus DNA sequences using primers corresponding to amino sequences that are common to all known protein kinases. One sequence was highly similar to KSS1, a MAP kinase from Saccharomyces cerevisiae. This sequence was used to isolate a full-length MAP kinase-like clone from a pea cDNA library. The pea clone, called D5, shared approximately 50% amino acid identity with MAP kinases from yeasts and vertebrates and about 41% identity with plant cdc2 kinases. An expression protein encoded by D5 was recognized by an antiserum specific to human MAP kinases (ERKs). Messenger RNA corresponding to D5 was present at similar levels in all tissues examined, without regard to whether cell division or elongation were occurring in those tissues.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00038997