Simple Capillary Electrophoretic Determination of Soluble Oxalate and Nitrate in Forage Grasses

A simple capillary electrophoretic method is described for the simultaneous determination of soluble oxalate and nitrate in forage grasses. Grass samples were ground and extracted with water. The extracts were filtered and submitted to capillary electrophoresis. Electrophoresis was performed in a 75...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2003-09, Vol.15 (5), p.480-483
Main Authors: Miyazaki, Shigeru, Yamanaka, Noriko, Guruge, Keerthi Siri
Format: Article
Language:English
Subjects:
absorption
Animal Feed - analysis
Animals
Biological and medical sciences
capillary electrophoresis
Electrophoresis, Capillary - methods
forage grasses
Fundamental and applied biological sciences. Psychology
Microbiology
monitoring
Nitrates - analysis
Oxalates - analysis
Poaceae - chemistry
Poaceae - classification
silica
sodium sulfate
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Summary:A simple capillary electrophoretic method is described for the simultaneous determination of soluble oxalate and nitrate in forage grasses. Grass samples were ground and extracted with water. The extracts were filtered and submitted to capillary electrophoresis. Electrophoresis was performed in a 75 μm ×50 cm fused silica capillary with 30 mM sodium sulfate containing an electroosmotic flow modifier under constant voltage at -8 kV Separated oxalate and nitrate were detected with direct UV absorption at 214 nm. The present method can be used for routine monitoring of the concentration of soluble oxalate and nitrate in grasses.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870301500516