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On-line SPE–CE for the determination of insulin derivatives in biological fluids

An on-line SPE–CE system is described for the determination of insulin derivatives in urine, serum and plasma. By combining techniques based on different separation mechanisms, in this case reversed-phase SPE and CE, a more selective sample clean-up is obtained. The described on-line SPE–CE procedur...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2003-10, Vol.33 (3), p.451-462
Main Authors: Visser, N.F.C, van Harmelen, M, Lingeman, H, Irth, H
Format: Article
Language:English
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Summary:An on-line SPE–CE system is described for the determination of insulin derivatives in urine, serum and plasma. By combining techniques based on different separation mechanisms, in this case reversed-phase SPE and CE, a more selective sample clean-up is obtained. The described on-line SPE–CE procedure is able to desalt and clean biological samples, resulting in more repeatable electrophoretic results as well as a good linearity for urine, serum and plasma samples spiked with insulin derivatives, thus proving the elimination of detrimental effects caused by the sample matrix. The on-line SPE–CE system was linear for urine, serum and plasma samples spiked with insulin derivatives between 5 and 80 mg/l. The repeatability in migration time was below 1% relative standard deviation (R.S.D.). The repeatability of the peak was better (
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(03)00292-9