On-line SPE–CE for the determination of insulin derivatives in biological fluids

• Published in: Journal of pharmaceutical and biomedical analysis 2003-10, Vol.33 (3), p.451-462
• Main Authors: Visser, N.F.C, van Harmelen, M, Lingeman, H, Irth, H
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Body Fluids - chemistry; Cattle; Electrophoresis, Capillary - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Insulin; Insulin - analysis; Insulin - genetics; Medical sciences; Molecular Sequence Data; On-line; Pharmacology. Drug treatments; Plasma; Serum; SPE; Technology, Pharmaceutical - methods; Urine
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(03)00292-9

An on-line SPE–CE system is described for the determination of insulin derivatives in urine, serum and plasma. By combining techniques based on different separation mechanisms, in this case reversed-phase SPE and CE, a more selective sample clean-up is obtained. The described on-line SPE–CE procedure is able to desalt and clean biological samples, resulting in more repeatable electrophoretic results as well as a good linearity for urine, serum and plasma samples spiked with insulin derivatives, thus proving the elimination of detrimental effects caused by the sample matrix. The on-line SPE–CE system was linear for urine, serum and plasma samples spiked with insulin derivatives between 5 and 80 mg/l. The repeatability in migration time was below 1% relative standard deviation (R.S.D.). The repeatability of the peak was better (