An AP2/EREBP-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein

• Published in: Theoretical and applied genetics 2003-10, Vol.107 (6), p.972-979
• Main Authors: CHEN, Jian-Quan, YI DONG, WANG, Yu-Jun, QIANG LIU, ZHANG, Jin-Song, CHEN, Shou-Yi
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; Amino acids; Analysis; Base Sequence; Biological and medical sciences; Classical and quantitative genetics. Population genetics. Molecular genetics; Cloning, Molecular; Cold Temperature; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Generalities. Genetics. Plant material; Genes; Genetic aspects; Genetic transcription; Genetics and breeding of economic plants; Genomes; Genomics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Oryza - cytology; Oryza - genetics; Oryza - metabolism; Plant Proteins; Protein Binding; Proteins; Recombinant Fusion Proteins - metabolism; Rice; Sequence Alignment; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription. Transcription factor. Splicing. Rna processing; Two-Hybrid System Techniques
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-003-1346-5

We cloned an AP2/EREBP gene by dot blotting and named it OsDREBL. Analysis of its deduced amino-acid sequence indicated that this protein had a potential nuclear-localization signal, a possible acidic-activation domain and an AP2 DNA binding domain. Northern analysis showed that the transcripts of OsDREBL accumulated rapidly (within 30 min) in response to low temperature, but not in response to ABA, NaCl and dehydration treatments. Southern analysis indicated the presence of a single-copy of the OsDREBL gene in the Oryza sativa genome. Our research also demonstrated that OsDREBL was localized to the nucleus but did not bind effectively to the C-repeat/dehydration responsive element (CRT/DRE). These results suggested that OsDREBL may function as a transcription factor in the cold-stress response, independent of the DREB signal-transduction pathway.