Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas

Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant ( hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in...

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Bibliographic Details
Published in:FEBS letters 2003-10, Vol.553 (1), p.109-112
Main Authors: Minoda, Ayumi, Sonoike, Kintake, Okada, Katsuhiko, Sato, Norihiro, Tsuzuki, Mikio
Format: Article
Language:English
Subjects:
Ammonium Hydroxide
Animals
Chlamydomonas
Chlamydomonas - drug effects
Chlamydomonas - genetics
Chlamydomonas - metabolism
Chlorophyll - metabolism
DCIP, 2,6-dichlorophenol indophenol
DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea
DPC, diphenylcarbazide
Electron Transport - drug effects
Glycolipid
Half-Life
Hydroxides - pharmacology
Kinetics
Light-Harvesting Protein Complexes
Mutation - genetics
PG, phosphatidylglycerol
Photosynthetic Reaction Center Complex Proteins - drug effects
Photosynthetic Reaction Center Complex Proteins - metabolism
Photosystem II
Photosystem II Protein Complex
PSII, photosystem II
Spectrometry, Fluorescence
SQDG, sulfoquinovosyl diacylglycerol
Sulfoquinovosyl diacylglycerol
Thylakoid membrane
Thylakoids - drug effects
Thylakoids - metabolism
Tyrosine - metabolism
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Summary:Photosystem (PS) II activity of a sulfoquinovosyl diacylglycerol (SQDG)-deficient mutant ( hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from Q A to Q B. This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)00981-5