A Sensitive Assay Method for the Measurement of S-Adenosylmethionine in Tissue

An assay method has been developed for the measurement of tissue levels of S-adenosylmethionine based upon the ability of this compound to activate tripoly-phosphatase associated with S-adenosylmethionine synthetase β prepared from rat liver. The method has been used to measure S-adenosylmethionine...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1984-01, Vol.96 (3), p.679-682
Main Authors: SUMA, Yukie, MATSUMOTO, Chiri, SAWAI, Yasuko, TSUKADA, Kinji
Format: Article
Language:English
Subjects:
Acid Anhydride Hydrolases
Animals
Biological and medical sciences
Biotechnology
Diet
Diverse techniques
Fundamental and applied biological sciences. Psychology
Kinetics
Liver - analysis
Liver - enzymology
Methionine - metabolism
Methionine Adenosyltransferase - metabolism
Methods. Procedures. Technologies
Molecular and cellular biology
Phosphoric Monoester Hydrolases - metabolism
Rats
Rats, Inbred Strains
S-Adenosylmethionine - analysis
Various methods and equipments
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An assay method has been developed for the measurement of tissue levels of S-adenosylmethionine based upon the ability of this compound to activate tripoly-phosphatase associated with S-adenosylmethionine synthetase β prepared from rat liver. The method has been used to measure S-adenosylmethionine levels in rat liver after feeding rats on various concentrations of methionine in the diet. The results obtained by this method agree well with those measured by the spectrophotometric method. The limit of sensitivity of the assay was about 0.1 nmol of S-adenosylmethionine in an incubation volume of 0.1 ml (10−6 M).
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a134885