Cultured Hepatoma Cells for the Study of Enzyme Regulation: Induction of Ornithine Decarboxylase by Insulin and Asparagine

The induction and decay of ornithine decarboxylase (ODC) by insulin and asparagine in cultures of H4-II-EC3 (H35) hepatoma cells was studied in a modified Waymouth medium in the presence of fetal bovine serum (FBS) and in serum-free media. The insulin response was enhanced by the presence of asparag...

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Bibliographic Details
Published in:In Vitro 1984-09, Vol.20 (9), p.723-731
Main Authors: Potter, Van R., Theresa Ruh Evanson, Debra P. Gayda, Gurr, James A.
Format: Article
Language:English
Subjects:
Amino acids
Animal cells
Animals
Asparagine - pharmacology
Biological and medical sciences
Cell cultures. Hybridization. Fusion
Cell Division
Cell growth
Cell lines
Cells, Cultured
Culture Media
Cultured cells
Enzyme Induction - drug effects
Fundamental and applied biological sciences. Psychology
Growth Substances - blood
Hepatocellular carcinoma
Hepatocytes
Inductive reasoning
Insulin
Insulin - pharmacology
Liver cells
Liver Neoplasms, Experimental - enzymology
Liver Neoplasms, Experimental - pathology
Molecular and cellular biology
Neurons
Ornithine Decarboxylase - biosynthesis
Putrescine - pharmacology
Rats
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Summary:The induction and decay of ornithine decarboxylase (ODC) by insulin and asparagine in cultures of H4-II-EC3 (H35) hepatoma cells was studied in a modified Waymouth medium in the presence of fetal bovine serum (FBS) and in serum-free media. The insulin response was enhanced by the presence of asparagine although the effect of asparagine was not so much on the initial increase as it was on a slowing of the decline after the maximum was reached at 6 to 8 h after the supplements were added together with fresh medium. In all cases the initial ODC activity was zero at zero time for addition of media and supplements, and, after reaching the maximum, activity declined to near zero by 24 h. Fetal bovine serum gave induction that followed a similar time course but was inferior to the combintion of insulin plus asparagine and, in fact, FBS inhibited the latter response. Putrescine (the product formed from ornithine by ODC), at 10-5M, markedly inhibited the induction of ODC by insulin or FBS, but the inhibition was less when asparagine was present.
ISSN:0073-5655
2327-4328
1475-2689
DOI:10.1007/BF02618878