Properties of carbodiimide treated heparin

Heparin, a well-known anticoagulant, has been frequently used to coat surfaces for attaining blood compatibility of polymeric materials. Since carbodiimides are often used for immobilization of heparin on these biomaterials, the present study intended to evaluate some properties of carbodiimide-trea...

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Bibliographic Details
Published in:Biomaterials 2003-11, Vol.24 (26), p.4777-4783
Main Authors: Oliveira, G.B., Carvalho, L.B., Silva, M.P.C.
Format: Article
Language:English
Subjects:
Biological Assay
Blood Coagulation - drug effects
Carbodiimide
Carbodiimides - chemistry
Carbodiimides - pharmacology
Cationic dyes
Heparin
Heparin - chemistry
Heparin - pharmacology
Heparin activities
Heparin Lyase - chemistry
IR spectra
Polysaccharide-Lyases - chemistry
Spectrophotometry, Infrared - methods
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Summary:Heparin, a well-known anticoagulant, has been frequently used to coat surfaces for attaining blood compatibility of polymeric materials. Since carbodiimides are often used for immobilization of heparin on these biomaterials, the present study intended to evaluate some properties of carbodiimide-treated heparin. It was observed that the properties of heparin were altered after treatment with carbodiimide, mainly in the molar excess of this reagent. Thus, dye fixation and electrophoretic behaviour of heparin were modified, as well as its degradability by specific enzymes. Also, these modifications resulted in loss of anticoagulant activity. Infrared spectra of this carbodiimide-treated heparin presented evidence that can confirm its modification.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(03)00376-4