Isolation of Rickettsia tsutsugamushi Antigenically Different from Kato, Karp, and Gilliam Strains from Patients

• Published in: MICROBIOLOGY and IMMUNOLOGY 1984, Vol.28(8), pp.873-882
• Main Authors: Tamura, Akira, Takahashi, Kumiko, Tsuruhara, Takashi, Urakami, Hiroshi, Miyamura, Sadao, Sekikawa, Hiroho, Kenmotsu, Minora, Shibata, Mitsuo, Abe, Shoya, Nezu, Hiroko
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Bacterial - immunology; Bacteriology; Biological and medical sciences; Cross Reactions; Fundamental and applied biological sciences. Psychology; Humans; L Cells (Cell Line) - microbiology; Mice; Microbiology; Orientia tsutsugamushi - immunology; Orientia tsutsugamushi - isolation & purification; Orientia tsutsugamushi - ultrastructure; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Rickettsia; Scrub Typhus - microbiology
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1984.tb00743.x

Rickettsia tsutsugamushi strains from three recent patients of Tsutsugamushi disease in Niigata Prefecture were isolated primarily in mice and then in L cell cultures. By this procedure, low virulent strains to mice, as well as high virulent ones, could be isolated and cultivated serially in L cell cultures, suggesting the usefulness of L cells for isolation of this species of rickettsia. Each newly isolated strain was identified as a member of R. tsutsugamushi from the results of cross immunological tests and morphological observation. On the other hand, it was recognized that one of these rickettsiae showed immunological properties distinguishable from the prototype strains of Kato, Karp, and Gilliam by the cross complement fixation test, and also had low virulence in mice.