Structure and expression of a barley acidic beta-glucanase gene

A barley acidic beta-1,3-glucanase gene was recovered from a barley genomic library by homology with a partial cDNA of barley basic beta-1,3-glucanase isoenzyme GII. The gene, Abg2, is homologous to the PR2 family of pathogenesis-related beta-1,3-glucanase genes. The ABG2 protein has 81% amino acid...

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Bibliographic Details
Published in:Plant molecular biology 1993-05, Vol.22 (2), p.347-360
Main Authors: Malehorn, D.E. (Monsanto Agricultural Group, St. Louis, MO (USA). New Products Div.), Scott, K.J, Shah, D.M
Format: Article
Language:English
Subjects:
Acids
Amino Acid Sequence
ARN MENSAJERO
ARN MESSAGER
Base Sequence
Biological and medical sciences
Cloning, Molecular
Escherichia coli - genetics
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
GENES
Genes, Plant - genetics
Genes. Genome
Genome
Glucan Endo-1,3-beta-D-Glucosidase - biosynthesis
Glucan Endo-1,3-beta-D-Glucosidase - genetics
Hordeum - enzymology
Hordeum - genetics
Hordeum - growth & development
HORDEUM VULGARE
ISOENZIMAS
ISOENZYME
ISOENZYMES
MESSENGER RNA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plant Proteins - genetics
Recombinant Proteins - biosynthesis
Restriction Mapping
RNA, Messenger - genetics
Seeds - enzymology
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tissue Distribution
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Summary:A barley acidic beta-1,3-glucanase gene was recovered from a barley genomic library by homology with a partial cDNA of barley basic beta-1,3-glucanase isoenzyme GII. The gene, Abg2, is homologous to the PR2 family of pathogenesis-related beta-1,3-glucanase genes. The ABG2 protein has 81% amino acid similarity to barley basic beta-1,3-glucanase GII. The ABG2 protein is encoded as a preprotein of 336 amino acids including a 28 amino acid signal peptide. A 299 bp intron occurs within codon 25. The mature ABG2 protein has a predicted mass of 32,642 Da and a calculated isoelectric point of 4.9. The second exon of the Abg2 gene shows a strong preference for G + C in the third position of degenerate codons. The Abg2 gene was functionally expressed in Escherichia coli. Abg2 mRNA is constitutively expressed in barley root; leaf expression of Abg2 mRNA is induced by mercuric chloride and infection by Erysiphe graminis f. sp. hordei. Southern blot analysis indicates that Abg2 is a member of a small gene family.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00014941