Stress responses in alfalfa (Medicago sativa L.). 15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family

We have identified five different full length chalcone synthase (CHS) cDNA clones from a cDNA library produced from transcripts isolated from an elicitor-treated alfalfa cell suspension culture. Nucleotide sequence similarity between the clones varied from 88-93%. Oligonucleotides based on divergent...

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Bibliographic Details
Published in:Plant molecular biology 1993-05, Vol.22 (2), p.239-253
Main Authors: Junghans, H. (Samuel Roberts Noble Foundation, Ardmore, OK (USA). Plant Biology Div.), Dalkin, K, Dixon, R.A
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
ADN
Amino Acid Sequence
Base Sequence
CALCONA
CHALCONE
CHALCONES
Cloning, Molecular
DNA
ESTRES
EXPRESION GENICA
EXPRESSION DES GENES
FEUILLE
GENE EXPRESSION
Gene Expression Regulation
Genome
HOJAS
LEAVES
MEDICAGO SATIVA
Medicago sativa - genetics
Molecular Sequence Data
Multigene Family - genetics
NUCLEOTIDE SEQUENCE
Oligonucleotide Probes
PHOMA
RACINE
RAICES
RNA, Messenger - genetics
ROOTS
SECUENCIA NUCLEICA
Sequence Analysis
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEIQUE
STRESS
Tissue Distribution
Transcription, Genetic
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Summary:We have identified five different full length chalcone synthase (CHS) cDNA clones from a cDNA library produced from transcripts isolated from an elicitor-treated alfalfa cell suspension culture. Nucleotide sequence similarity between the clones varied from 88-93%. Oligonucleotides based on divergent sequences in the 5'-untranslated regions of the clones could distinguish individual genes, or groups of genes, and their corresponding transcripts. Developmentally regulated expression of the CHS transcripts was predominantly in roots and root nodules; other unidentified members of the CHS gene family are expressed in stems, leaves and nodules. One of the CHS transcripts was strongly expressed in floral tissue. All the CHS transcripts studied were induced in elicitor-treated cell suspension cultures. Transcripts were also induced in roots in response to wounding or spraying with various elicitors, and in leaves infected with Phoma medicaginis (but not in wounded leaves). The induction kinetics of CHS2 transcripts were more rapid and/or transient than those of other members of the CHS family in CuCl2-treated roots and Phoma-infected leaves. The results are discussed in terms of the evolution and functions of the CHS gene family in legumes.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00014932