Expression of active human factor VIII from recombinant DNA clones

DNA clones encoding the complete 2,351 amino acid sequence for human factor VIII have been isolated and used to produce biologically active factor VIII in cultured mammalian cells. The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and imm...

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Bibliographic Details
Published in:Nature (London) 1984-01, Vol.312 (5992), p.330-337
Main Authors: Wood, William I, Capon, Daniel J, Simonsen, Christian C, Eaton, Dan L, Gitschier, Jane, Keyt, Bruce, Seeburg, Peter H, Smith, Douglas H, Hollingshead, Philip, Wion, Karen L, Delwart, Eric, Tuddenham, Edward G. D, Vehar, Gordon A, Lawn, Richard M
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Blood coagulation. Blood cells
Cells, Cultured
clones
cloning
Cloning, Molecular
coagulation factor VIII
Coagulation factors
Cricetinae
DNA
DNA - genetics
DNA, Recombinant
Factor VIII - genetics
Female
Fundamental and applied biological sciences. Psychology
gene expression
Gene Expression Regulation
Genes
Health. Pharmaceutical industry
Hemophilia A - therapy
Humanities and Social Sciences
Humans
Industrial applications and implications. Economical aspects
mammalian cells
man
Molecular and cellular biology
Molecular Sequence Data
multidisciplinary
Other active biomolecules
Production of active biomolecules
RNA, Messenger - genetics
Science
Science (multidisciplinary)
X Chromosome
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Description
Summary:DNA clones encoding the complete 2,351 amino acid sequence for human factor VIII have been isolated and used to produce biologically active factor VIII in cultured mammalian cells. The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.
ISSN:0028-0836
1476-4687
DOI:10.1038/312330a0