Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation

How eukaryotic promoter-specific activator proteins (activators) stimulate transcription is a central question. We have previously shown that an acidic activator can directly interact with the general transcription factor TFIIB and increase its stable assembly into a preinitiation complex. We have p...

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Bibliographic Details
Published in:Nature (London) 1993-06, Vol.363 (6431), p.741-744
Main Authors: Roberts, Stefan G. E, Ha, Ilho, Maldonado, Edio, Reinberg, Danny, Green, Michael R
Format: Article
Language:English
Subjects:
Binding Sites
Biological and medical sciences
Cloning, Molecular
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Fungal Proteins - metabolism
Glutathione Transferase - genetics
HeLa Cells
Herpes Simplex Virus Protein Vmw65 - metabolism
Humans
Molecular and cellular biology
Molecular genetics
Mutation
Protein Binding
Proteins
Recombinant Fusion Proteins - metabolism
Ribonucleic acid
RNA
Trans-Activators - metabolism
Transcription Factor TFIIB
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
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Summary:How eukaryotic promoter-specific activator proteins (activators) stimulate transcription is a central question. We have previously shown that an acidic activator can directly interact with the general transcription factor TFIIB and increase its stable assembly into a preinitiation complex. We have proposed that this increase in TFIIB assembly is at least part of the mechanism by which an acidic activator functions. A prediction of this hypothesis is that a TFIIB mutant unable to interact with an acidic activator could not support activated transcription, and here we present experiments that verify this prediction. In conjunction with previous studies, our results argue that interaction between an acidic activator and TFIIB is necessary for transcriptional activation.
ISSN:0028-0836
1476-4687
DOI:10.1038/363741a0