Purified Human Granulocyte-Macrophage Colony-Stimulating Factor: Direct Action on Neutrophils

Neutrophil migration inhibition factor from T lymphocytes (NIF-T) is a lymphokine that acts to localize granulocytes. Medium conditioned by the Mo human T-lymphoblast cell line was used to purify NIF-T, a glycoprotein with a molecular weight of 22,000. The NIF-T was found to potently stimulate the g...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1984-12, Vol.226 (4680), p.1339-1342
Main Authors: Gasson, Judith C., Weisbart, Richard H., Kaufman, Susan E., Clark, Steven C., Hewick, Rodney M., Wong, Gordon G., Golde, David W.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Biological and medical sciences
Bone marrow
Bone Marrow Cells
Cell Division
Cell interactions
Cell Line
Cell lines
Chromatography, High Pressure Liquid
Colony-Stimulating Factors - isolation & purification
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gels
Granulocyte macrophage progenitor cells
Granulocytes
Granulocytes - cytology
Humans
Immunobiology
Immunologic research
Immunological research
Legends
Leukemia research
Leukocyte Migration-Inhibitory Factors - pharmacology
Lymphocytes
Lymphokines - pharmacology
Macrophages - cytology
Molecular Weight
Neutrophils
Oncology
Stem cells
T lymphocytes
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Summary:Neutrophil migration inhibition factor from T lymphocytes (NIF-T) is a lymphokine that acts to localize granulocytes. Medium conditioned by the Mo human T-lymphoblast cell line was used to purify NIF-T, a glycoprotein with a molecular weight of 22,000. The NIF-T was found to potently stimulate the growth of granulocyte and macrophage colonies from human bone marrow and colony formation by the KG-1 myeloid leukemia cell line. Thus a human lymphokine (NIF-T) that modulates the activities of mature neutrophilic granulocytes is also a colony-stimulating factor acting on precursors to induce growth and differentiation of new effector cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.6390681