Commitment of Cell Fate in the Early Zebrafish Embryo

When do single cells in the early zebrafish embryo become irreversibly committed to a specific fate? Work with lineage tracing and fate mapping has shown that the marginal cells of the blastoderm give rise to hypoblast-derived fates (mesoderm and endoderm). However, experiments described here show t...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1993-07, Vol.261 (5117), p.109-111
Main Authors: Ho, Robert K., Kimmel, Charles B.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blastocyst - cytology
Blastoderm - cytology
Cell Movement
Cell transplantation
Cellular biology
Developmental cytology
Developmental Stages
Donors
Early stages. Segmentation. Gastrulation. Neurulation
Embryogenesis
Embryology: invertebrates and vertebrates. Teratology
Embryonic stem cells
Embryos
Endothelial cells
Fish
Fishes
Freshwater
Fundamental and applied biological sciences. Psychology
Gastrula - cytology
Growth
Individualized Instruction
Mesenchymal stem cells
Neurons
Pluripotent stem cells
Reproduction
Stem Cell Transplantation
Stem cells
Stem Cells - cytology
Transplantation, Heterotopic
Zebrafish - embryology
Zoology
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Summary:When do single cells in the early zebrafish embryo become irreversibly committed to a specific fate? Work with lineage tracing and fate mapping has shown that the marginal cells of the blastoderm give rise to hypoblast-derived fates (mesoderm and endoderm). However, experiments described here show that these marginal blastoderm cells remain pluripotent and uncommitted throughout the late blastula and early gastrula stages. Embryonic cells become committed to a hypoblast-derived fate at mid-gastrulation. Timelapse photographic analysis reveals that committed cells, when transplanted heterotopically and heterochronically, can migrate along atypical pathways to reposition themselves within a more correct environment.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8316841