Physical mapping in the region of human Xq12-21.1 using pulsed field gel electrophoresis

A number of human disease genes have been localised to Xq12-21.1. A genetic map of this region has previously been constructed using family linkage studies and has been complemented by physical mapping studies using hybrid and deletion cell lines. We have constructed a preliminary long-range physica...

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Bibliographic Details
Published in:Human genetics 1993-06, Vol.91 (5), p.485-488
Main Authors: JONES, A. M, MALCOLM, S, LEVINSKY, R. J, KINNON, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromosome Mapping - methods
Classical genetics, quantitative genetics, hybrids
DNA Probes
Electrophoresis, Gel, Pulsed-Field
Female
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
Polymorphism, Genetic
X Chromosome
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Summary:A number of human disease genes have been localised to Xq12-21.1. A genetic map of this region has previously been constructed using family linkage studies and has been complemented by physical mapping studies using hybrid and deletion cell lines. We have constructed a preliminary long-range physical map of the region, which incorporates thirteen polymorphic and non-polymorphic probes, using pulsed field gel electrophoresis. The order of loci that can be inferred from all the genetic and physical mapping data is: cen-DXS133-[DXS153, DXS159]-DXS132-DXS135-[DXS131, DXS162]-[DXS325, DXS-347, DXS441]-PGK1-DXS447-DXS72-tel. The detection of several large non-overlapping MluI fragments by these probes implies that the minimum extent of the genomic DNA containing these loci is 16 Mb. This information should be useful in the eventual identification and isolation of the genes responsible for diseases that map to this region.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00217777