Vitronectin Secretion by Hepatic and Non-Hepatic Human Cancer Cells

Thirty-eight human cancer cell lines and subclones derived from 12 different organs were screened for vitronectin secretion in their culture media. By immunoblotting analysis we detected high secretion by three out of five hepatoma cell lines tested but no secretion by the others. In addition, signi...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology - Animal 1993-05, Vol.29A (5), p.403-407
Main Authors: Yasumitsu, Hidetaro, Noriko Seo, Emi Misugi, Hatsué Morita, Miyazaki, Kaoru, Umeda, Makoto
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Biotechnology
Blotting, Western
Cell Adhesion
Cell Line
Cell lines
Cells
Electrophoresis, Polyacrylamide Gel
Eukaryotic cell cultures
Extracellular Matrix Proteins - biosynthesis
Fundamental and applied biological sciences. Psychology
Glycoproteins - analysis
Glycoproteins - biosynthesis
Glycoproteins - secretion
Growth, Differentiation and Senescence
HeLa Cells
Hepatocellular carcinoma
Hepatocytes
Humans
KB cells
Liver
Liver - metabolism
Methods. Procedures. Technologies
Miscellaneous
Secretion
Tumor cell line
Tumor Cells, Cultured - metabolism
Tumors
Vitronectin
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Summary:Thirty-eight human cancer cell lines and subclones derived from 12 different organs were screened for vitronectin secretion in their culture media. By immunoblotting analysis we detected high secretion by three out of five hepatoma cell lines tested but no secretion by the others. In addition, significant secretion was observed in seven non-hepatic cancer cell lines and subclones derived from the cervix, lung, and pancreas. These vitronectin-secreting cells included PLC/PRF/5, HuH-6 #5, HuH-7, HeLa S3, HeLa · P3 #2, #3, #6, #8, A549, and MIAPaCa-2. The results were further confirmed by quantitative analysis using sandwich enzyme-linked immunoassay, and activity analysis of cell attachment promotion on Western blotted filters.
ISSN:1071-2690
0883-8364
1543-706X
2327-431X
DOI:10.1007/BF02633989